The Repro-prophylactic Impact of Synthetic Tocopherol Polyethylene Glycol, Succinate-Coated, Garlic-Selenium Nanoparticles against Lead Acetate Toxicity in Male Rabbits

Authors

  • Mohamed A. R. Yassen Faculty of Science of Sfax, University of Sfax, BP 1171, 3000 Sfax, Tunisia.
  • Madiha Hadj-Ayed Institude Supérieur Agronomique de Chott-Mariem, University of Sousse, Tunisia. https://orcid.org/0000-0002-2900-8749
  • Jabbar A. A. Al-Saaidi Department of Basic Sciences, College of Dentistry, Al-Ayen University, Nasiriyah, Iraq. https://orcid.org/0000-0003-2022-2287

DOI:

https://doi.org/10.21123/bsj.2024.11311

Keywords:

Lead acetate, Reproduction, Selenium, Tocopherol, Testes, Toxicity.

Abstract

Because of industrial and human activities, lead is a hazardous heavy metal that is widely present in the environment and poses a threat to public health and ecosystems. Garlic extract (GE) demonstrates protective impacts against many disorders. Its effects on heavy metal toxicity require more research. A nanoparticle formulation could increase its effectiveness even more. This investigation examined the repro-protection impact of tocopherol polyethylene glycol succinate-coated garlic selenium (TPGS-GSNP) against lead acetate (LA) toxicity. For this purpose, 64 mature male rabbits were equally divided into eight groups (8 each). Male rabbits were treated three times a week for 12 weeks. The males received drinking water (G1), 30 mg of LA/ kg. BW (G2), 800 mg of GE/kg. BW (G3), GE and LA (G4), 1 mg of TPGS-Selenium/kg. BW (G5), TPGS-S and LA (G6), 1 mg of TPGS-GSNP/kg. BW (G7), and TPGS-GSNP and LA (G8). After treatment, serum levels of follicle stimulating hormone, luteinizing hormone, testosterone, and gonadotropin releasing hormone were assessed. Testicular histopathological findings and the expression levels of testicular FSH and LH receptors, ABP, 3β-HSD, 17β-HSD, and Inh-α, and pituitary LHβ and FSHβ genes were evaluated. Obvious testicular histopathological changes, decreased genital organ weights, reduced serum hormonal levels, and declined testicular and pituitary gene expression levels, were exhibited in LA group. The TPGS-GSNP group, however, displayed improvement in these findings. Finally, synthesized TPGS-GSNP showed a greater repro-protective effect against LA toxic-induced male rabbits. Further research is required to explore the mechanism of TPGS-GSNP action.

References

Abdussalam-Mohammed WJ. Review of therapeutic applications of nanotechnology in medicine field and its side effects. J Chem Rev. 2019; 1(3): 243-51. https://doi.org/10.33945/SAMI/JCR.2019.3.5

Harish V, Tewari D, Gaur M, Yadav AB, Swaroop S, Bechelany M, et al. Review on nanoparticles and nanostructured materials: Bioimaging, biosensing, drug delivery, tissue engineering, antimicrobial, and agro-food applications. Nanomater. 2022 Jan 28; 12(3): 457-499. https://doi.org/10.3390/nano12030457

Othman MS, Obeidat ST, Aleid GM, Abdel-Daim MM, Habotta OA, Schwartz L. et al. Pergularia tomentosa coupled with selenium nanoparticles salvaged lead acetate-induced redox imbalance, inflammation, apoptosis, and disruption of neurotransmission in rats’ brain. Open Chem. 2022 Nov 23; 20(1): 1313-26. https://doi.org/10.1515/chem-2022-0246.

Abdel‐Emam RA, Ahmed EA. Ameliorative effect of L‐carnitine on chronic lead‐induced reproductive toxicity in male rats. Vet Med Sci. 2021 Jul; 7(4): 1426-35. https://doi.org/10.1002/vms3.473.

El‐Magd MA, Kahilo KA, Nasr NE, Kamal T, Shukry M, Saleh AA. A potential mechanism associated with lead‐induced testicular toxicity in rats. Andrologia. 2017 Nov; 49(9): e12750. https://doi.org/10.1111/and.12750

Kucukler S, Benzer F, Yildirim S, Gur C, Kandemir FM, Bengu AS, et al. Protective effects of chrysin against oxidative stress and inflammation induced by lead acetate in rat kidneys: a biochemical and histopathological approach. Biol Trace Elem Res. 2021 Apr; 199(4): 1501-14. https://doi.org/10.1007/s12011-020-02268-8

Hosny NS, Hashem NM, Morsy AS, Abo-Elezz ZR. Effects of organic selenium on the physiological response, blood metabolites, redox status, semen quality, and fertility of rabbit bucks kept under natural heat stress conditions. Front Vet Sci. 2020 Jun 12; 7: 290.45-46. https://doi.org/10.3389/fvets.2020.00290

Alharthi WA, Hamza RZ, Elmahdi MM, Abuelzahab HS, Saleh H. Selenium and L-carnitine ameliorate reproductive toxicity induced by cadmium in male mice. Biol Trace Elem Res. 2020 Oct; 197(2): 619-27. https://doi.org/10.1007/s12011-019-02016-7

Hamza RZ, Diab AE. Testicular protective and antioxidant effects of selenium nanoparticles on Monosodium glutamate-induced testicular structure alterations in malemice. Toxicol Rep. 2020Jan1; 7: 254-60. https://doi.org/10.1016/j.toxrep.2020.01.012

Faponle AS, Osonuga IO, Ezima EN, Adenowo TK, Adelegan AA. Effect of aqueous extract of Allium sativum (Garlic) on fertility in male Wistar rats. Ann Health Res. 2020 Mar 28; 6(1): 100-7. https://doi.org/10.30442/ahr.0601-11-71

Bazaraliyeva A, Moldashov D, Turgumbayeva A, Kartbayeva E, Kalykova A, Sarsenova L, et al. Chemical and biological properties of bio-active compounds from garlic (Allium sativum). Pharmacia. 2022; 69(4): 955-64. https://doi.org/10.3897/pharmacia.93.e93604.

El-Saber Batiha G, Magdy Beshbishy A, G. Wasef L, Elewa YH, A. Al-Sagan A, Abd El-Hack ME, ,et al. Chemical constituents and pharmacological activities of garlic (Allium sativum L.): A review. Nutrients. 2020 Mar 24; 12(3): 872-892. https://doi.org/10.3390/nu12030872

Galli F, Bonomini M, Bartolini D, Zatini L, Reboldi G, Marcantonini G, et al. Vitamin E (alpha-tocopherol) metabolism and nutrition in chronic kidney disease. Antioxidants. 2022 May 18; 11(5): 989-1007. https://doi.org/10.3390/antiox11050989

Shen Y, Liu K, Luo X, Cheng L. The low prevalence rate of vitamin E deficiency in urban adults of Wuhan from central China: findings from a single-center, cross-sectional study. Eur J Med Res. 2023 Mar 30; 28(1): 141-150. https://doi.org/10.1186/s40001-023-01103-9

Ahmed YF, Mahmoud GH, Farghaly AA, Abo-Zeid MA, Ismail EM. Some studies on the toxic effects of prolonged lead exposure in male rabbits: chromosomal and testicular alterations. Glob Vet. 2012 Jun 13; 8(4): 360-6.

Daher MH, Muraih JK. Influence of Capparis Spinosa Crude Extract on the Amylase Activity in Vitro. Ann Rom Soc Cell Biol. 2021 Mar 1: 2410-5.

Redfern J, Kinninmonth M, Burdass D, Verran J. Using soxhlet ethanol extraction to produce and test plant material (essential oils) for their antimicrobial properties. J Microbiol Biol Educ. 2014 May; 15(1): 45-6. https://doi.org/10.1128/jmbe.v15i1.656

Al-Shreefy HH, Al-Awady MJ, Al-Wasiti E. Characterization and cytotoxicity of novel synthesis selenium nanoparticles stabilized by vitamin E TPGS. J Univ Shanghai Sci Technol. 2022; 24(2): 214-38.

Saeid A, editor. Food biofortification technologies. CRC Press; 2017 Nov 22. https://doi.org/10.1201/9781351228350

Sheweita SA, El-Dafrawi YA, El-Ghalid OA, Ghoneim AA, Wahid A. Antioxidants (selenium and garlic) alleviated the adverse effects of tramadol on the reproductive system and oxidative stress markers in male rabbits. Sci Rep. 2022 Aug 17; 12(1): 13958. https://doi.org/10.1038/s41598-022-16862-4

Li H, Yan L, Tang EK, Zhang Z, Chen W, Liu G, Mo J. Synthesis of TPGS/curcumin nanoparticles by thin-film hydration and evaluation of their anti-colon cancer efficacy in vitro and in vivo. Front pharmacol. 2019 Jul 12; 10: 447207. https://doi.org/10.3389/fphar.2019.00769

Khoshnamvand M, Huo C, Liu J. Silver nanoparticles synthesized using Allium ampeloprasum L. leaf extract: characterization and performance in catalytic reduction of 4-nitrophenol and antioxidant activity. J Mol Struct. 2019Jan5; 1175: 90-6. https://doi.org/10.1016/j.molstruc. 2018.07.089

Mourdikoudis S, Pallares RM, Thanh NT. Characterization techniques for nanoparticles: comparison and complementarity upon studying nanoparticle properties. Nanoscale. 2018; 10(27): 12871-934. https://doi.org/10.1039/C8NR02278J

Burow S, Fontaine R, von Krogh K, Mayer I, Nourizadeh-Lillabadi R, Hollander-Cohen L, et al. Follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh): Developmental profiles of pituitary protein and gene expression levels. Gen Comp Endocrinol. 2019 Feb1; 272: 93-108.

Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods. 2001 Dec 1; 25(4): 402-8.

Bilinska B, Hejmej A, Kotula-Balak M. Preparation of testicular samples for histology and immunohistochemistry. Sertoli Cells: Methods Protoc. 2018: 17-36. https://doi.org/10.1007/978-1-4939-7698-0_3

Piaggesi A, Viacava P, Rizzo L, Naccarato G, Baccetti F, Romanelli M, et al. Semiquantitative analysis of the histopathological features of the neuropathic foot ulcer: effects of pressure relief. Diabetes care. 2003 Nov 1; 26(11): 3123-8. https://doi.org/10.2337/diacare.26.11.3123

Schefler, HG. Statistics for the biological sciences. Addison—Wesley Publishing Company, London 1969. 232 S., 42 Tab., 12 Lit., Preis 65/—. https://doi.org/10.1002/bimj.19700120316

Albarakati AJ, Baty RS, Aljoudi AM, Habotta OA, Elmahallawy EK, Kassab RB, Abdel Moneim AE. Luteolin protects against lead acetate-induced nephrotoxicity through antioxidant, anti-inflammatory, anti-apoptotic, and Nrf2/HO-1 signaling pathways. Mol Biol Rep. 2020 Apr; 47(4): 2591-603. https://doi.org/10.1007/s11033-020-05346-1

Ibrahim IA, Shalaby AA, Abd Elaziz RT, Bahr HI. Chlorella vulgaris or Spirulina platensis mitigate lead acetate-induced testicular oxidative stress and apoptosis with regard to androgen receptor expression in rats. Environ Sci Pollut Res Int. 2021 Aug; 28(29): 39126-38. https://doi.org/10.1007/s11356-021-13411-w

Xue W, Tang Q, Yang L. The combination of ginger and zinc supplement could improve lead‐induced reproductive dysfunction by inhibiting apoptosis mediated by oxidative damage and inflammation. Andrologia. 2022 Dec; 54(11): e14577. https://doi.org/10.1111/and.14577

Abdel-Wareth AA, Ahmed AE, Hassan HA, Abd El-Sadek MS, Ghazalah AA, Lohakare J. Nutritional impact of nano-selenium, garlic oil, and their combination on growth and reproductive performance of male Californian rabbits. Anim Feed Sci Technol. 2019 Mar 1; 249: 37-45. https://doi.org/10.1016/j.anifeedsci.2019.01.016.

Thomson M, Al-Qattan KK, Js D, Ali M. Anti-diabetic and anti-oxidant potential of aged garlic extract (AGE) in streptozotocin-induced diabetic rats. BMC Complement Altern Med. 2015 Dec; 16: 1-9. https://doi.org/10.1186/s12906-016-0992-5

Sokol RZ, Wang S, Wan YJ, Stanczyk FZ, Gentzschein E, Chapin RE. Long-term, low-dose lead exposure alters the gonadotropin-releasing hormone system in the male rat. EHP. 2002 Sep; 110(9): 871-4. https://doi.org/10.1289/ehp.02110871

Wahab OA, Princely AC, Oluwadamilare AA, Oore-oluwapo DO, Blessing AO, Alfred EF. Clomiphene citrate ameliorated lead acetate-induced reproductive toxicity in male Wistar rats. JBRA Assist Reprod. 2019 Oct; 23(4): 336-343. https://doi.org/10.5935/1518-0557.20190038

Owumi SE, Arunsi UO, Otunla MT, Oluwasuji IO. Exposure to lead and dietary furan intake aggravates hypothalamus-pituitary-testicular axis toxicity in chronic experimental rats. J Biomed Res. 2023 Mar; 37(2): 100. https://doi.org/10.7555/JBR.36.20220108

Soleimanzadeh A, Mohammadnejad L, Ahmadi A. Ameliorative effect of Allium sativum extract on busulfan-induced oxidative stress in mice sperm. Vet Res Forum. 2018 Summer; 9(3): 265-271. https://doi.org/10.30466/vrf.2018.32079 . Epub 2018 Sep 15. PMID: 30357081; PMCID: PMC6198159.

Monsees TK, Winterstein U, Hayatpour J, Schill WB, Miska W. Effect of heavy metals on the secretory function of testicular cells in culture. J Trace Microprobe Tech. 1998 Jan 1; 16(4): 427-35.

Segaloff DL. Regulatory processes governing the cell surface expression of LH and FSH receptors. Subcell Biochem. 2012; 63: 113-29. https://doi.org/10.1007/978-94-007-4765-4_7 . PMID: 23161136

Abdrabou MI, Elleithy EM, Yasin NA, Shaheen YM, Galal M. Ameliorative effects of Spirulina maxima and Allium sativum on lead acetate-induced testicular injury in male albino rats with respect to caspase-3 gene expression. Acta Histochemica. 2019 Feb 1; 121(2): 198-206. https://doi.org/10.1016/j.acthis.2018.12.006.

Asadpour R, Aliyoldashi MH, Saberivand A, Hamidian G, Hejazi M. Ameliorative effect of selenium nanoparticles on the structure and function of testis and in vitro embryo development in Aflatoxin B1‐exposed male mice. Andrologia. 2020 Dec; 52(11): e13824. https://doi.org/10.1111/and.13824

Khoshbakht S, Motejaded F, Karimi S, Jalilv N, Ebrahimzadeh-Bideskan A. Protective effects of selenium on electromagnetic field-induced apoptosis, aromatase P450 activity, and leptin receptor expression in rat testis. Iran J Basic Med Sci. 2021 Mar; 24(3): 322-330. https://doi.org/10.22038/ijbms.2021.45358.10554

Downloads

Issue

Section

article

How to Cite

1.
The Repro-prophylactic Impact of Synthetic Tocopherol Polyethylene Glycol, Succinate-Coated, Garlic-Selenium Nanoparticles against Lead Acetate Toxicity in Male Rabbits. Baghdad Sci.J [Internet]. [cited 2024 Nov. 6];22(5). Available from: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/11311