The Repro-prophylactic Impact of Synthetic Tocopherol Polyethylene Glycol, Succinate-Coated, Garlic-Selenium Nanoparticles against Lead Acetate Toxicity in Male Rabbits
DOI:
https://doi.org/10.21123/bsj.2024.11311الكلمات المفتاحية:
خلات الرصاص، الخصى، الجسيمات النانوية، التكاثر، السلينيوم، توكوفيرول، التسمم.الملخص
بسبب النشاطات الصناعية والبشرية ، يعد الرصاص من المعادن الثقيلة الخطرة الموجودة على نطاق واسع في البيئة، ويشكل تهديدا للصحة العامة والنظم البيئية. يظهر مستخلص الثوم أهمية وقائية ضد العديد من الاضطرابات الوظيفية، وعليه تتطلب دراسة تأثيره ضد سمية المعادن الثقيلة المزيد من البحوث. بإمكان الجسيمات النانوية المصنعة الحاوية على مركبات المستخلص أن تكون أكثر فعالية. تضمنت الدراسة الحالية التحري عن دور الجسيمات النانوية المصنعة من التوكوفيرول كلايكول –ساكسينيت المغلف للثوم والسلينيوم (TPGS-GSNP) لوقاية الجهاز التكاثري من التسمم بالرصاص. لتحقيق أهداف الدراسة، تم توزيع 64 أرنبا ذكرا ناضجا بالتساوي على ثمان مجموعات. عوملت الذكور ثلاث مرات اسبوعيا ولمدة 12 اسبوع. عوملت المجموعات بالماء المقطر (G1)، 30 ملغم من خلات الرصاص/كغم من وزن الجسم (G2)، 800 ملغم من خلاصة الثوم/ كغم من وزن الجسم (G3)، خلات الرصاص وخلاصة الثوم معا (G4)، 22.13 ملغم من مركب TPGS مع السلينيوم (G5)، خلات الرصاص و مركب TPGS مع السلينيوم (G6)، 22.13 ملغم من الجسيمات النانوية TPGS-GSNP/ كغم من وزن الجسم (G7)، خلات الرصاص و الجسيمات النانوية TPGS-GSNP (G8). بعد انتهاء المعاملة، تم قياس مستوى الهرمون المحفز للجريب والهرمون المصفر و التسيوستيرون و الهرمون المحرر لمحرضات القند. تم تقييم المعطيات النسجية المرضية للخصى و مستوى تعبير جينات FSHβ و LHβ في النخامية وجينات مستقبلات FSH و LH و جينات ABP و 3β-HSD و 17β-HSD و Inh-α في الخصى. أظهرت المجموعة المعاملة بخلات الرصاص تغيرات نسجية مرضية واضحة مع انخفاض ملحوظ في مستوى الهرمونات ومستوى تعبير الجينات للنخامية والخصى. أما المعاملة بالجسيمات النانوية TPGS-GSNP مع أو بدون خلات الرصاص، فقد أظهرت تحسنا ملحوظا. يستنتج أن للجسيمات النانوية TPGS-GSNP المصنعة تأثيرا كبيرا ضد آثار التسمم بالرصاص على الجهاز التكاثري المشتحث في ذكور الأرانب الناضجة. الموضوع يتطلب المزيد من البحوث لاستكشاف آلية عمل الجسيمات النانوية TPGS-GSNP.
Received 28/03/2024
Revised 11/06/2024
Accepted 13/06/2024
Published Online First 20/10/2024
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