The Repro-prophylactic Impact of Synthetic Tocopherol Polyethylene Glycol, Succinate-Coated, Garlic-Selenium Nanoparticles against Lead Acetate Toxicity in Male Rabbits
DOI:
https://doi.org/10.21123/bsj.2024.11311Keywords:
Lead acetate, Reproduction, Selenium, Tocopherol, Testes, Toxicity.Abstract
Because of industrial and human activities, lead is a hazardous heavy metal that is widely present in the environment and poses a threat to public health and ecosystems. Garlic extract (GE) demonstrates protective impacts against many disorders. Its effects on heavy metal toxicity require more research. A nanoparticle formulation could increase its effectiveness even more. This investigation examined the repro-protection impact of tocopherol polyethylene glycol succinate-coated garlic selenium (TPGS-GSNP) against lead acetate (LA) toxicity. For this purpose, 64 mature male rabbits were equally divided into eight groups (8 each). Male rabbits were treated three times a week for 12 weeks. The males received drinking water (G1), 30 mg of LA/ kg. BW (G2), 800 mg of GE/kg. BW (G3), GE and LA (G4), 1 mg of TPGS-Selenium/kg. BW (G5), TPGS-S and LA (G6), 1 mg of TPGS-GSNP/kg. BW (G7), and TPGS-GSNP and LA (G8). After treatment, serum levels of follicle stimulating hormone, luteinizing hormone, testosterone, and gonadotropin releasing hormone were assessed. Testicular histopathological findings and the expression levels of testicular FSH and LH receptors, ABP, 3β-HSD, 17β-HSD, and Inh-α, and pituitary LHβ and FSHβ genes were evaluated. Obvious testicular histopathological changes, decreased genital organ weights, reduced serum hormonal levels, and declined testicular and pituitary gene expression levels, were exhibited in LA group. The TPGS-GSNP group, however, displayed improvement in these findings. Finally, synthesized TPGS-GSNP showed a greater repro-protective effect against LA toxic-induced male rabbits. Further research is required to explore the mechanism of TPGS-GSNP action.
Received 28/03/2024
Revised 11/06/2024
Accepted 13/06/2024
Published Online First 20/10/2024
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