الملف الكيميائي النباتي، مضادات الأكسدة، مثبط الإنزيم ونشاط السمية الحادة من Astragalus bruguieri
DOI:
https://doi.org/10.21123/bsj.2022.6769الكلمات المفتاحية:
السمية الحادة، مضادات الأكسدة، استراغالوس بروغيري، مثبطات الإنزيم، كيمياء النباتالملخص
تم استخدام النباتات الطبية (أنواع استراغالوس) تقليديًا كمضاد للالتهابات ومضاد للأكسدة ومضاد لمرضى السكر. يبحث البحث الحالي في الكيمياء النباتية وبعض النشاط البيولوجي لمستخلص الميثانول لأجزاء مختلفة من Astragalus bruguieri Bioss.، وهو نبات طبي بري ينمو في جبل سفين، أربيل، العراق. تم تحليل المستخلصات الميثانولية للنبات A. bruguieri لمعرفة محتويات الفينول والفلافونويد والسابونين. تم تحليل نشاط مضادات الأكسدة في المختبر بواسطة مقايسات 2،2-diphenyl-1-picrylhydrazyl (DPPH) و2،2'azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). علاوة على ذلك، تم فحص المستخلصات النباتية لمعرفة النشاط المثبط للإنزيم المختبري والسمية شبه الحادة في الجسم الحي. أظهرت النتائج أعلى محتوى إجمالي من الفينول (28.83، 20.62 مجم GAEs / جم) في الأوراق والجذور على التوالي. بينما وجد أعلى محتوى إجمالى من الفلافونويد (50.08، 44.01 مجم REs / جم) في الأجزاء الهوائية والأوراق على التوالي. كان إجمالي الصابونين أكثر انتشارًا (25.33، 23.18 مجم من مستخلص GAEs / جم) في الجذور والأجزاء الهوائية، على التوالي. أظهر قياس نشاط مضادات الأكسدة في المختبر بواسطة مقايسة (DPPH) أن الأوراق كجزء متفوق في هذا النشاط (42.19 mg مستخلص TEs / g)، بينما أشار تقييم مضادات الأكسدة بواسطة مقايسة (ABTS) إلى أن الجذور هي الجزء الأكثر نشاطًا (86.90 mg TEs / مستخلص ز). تم العثور على α-glucosidase وα-amylase لتكون 0.45-0.67 و1.2-1.8 مللي مول ACAEs / جم على التوالي. أشار اختبار السمية الفموية الحادة إلى سلامة جرعة 600 مجم / كجم لأجزاء مختلفة من A. bruguieri على الجرذان البيضاء دون حدوث خلل سلوكي أو الموت. تعتبر الدراسة الحالية أول تقرير عن A. bruguieri كمصدر جديد محتمل للمواد المتوافقة حيويًا للعديد من المنتجات الصناعية.
Received 20/11/2021
Accepted 22/3/2022
Published Online First 20/7/2022
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