التأثير التآزري لـ Weisella cibaria والجسيمات النانوية الحيوية من Fusarium oxysporum ضد خلايا سرطان عنق الرحم
DOI:
https://doi.org/10.21123/bsj.2023.7956الكلمات المفتاحية:
الكلمات المفتاحية: سرطان عنق الرحم، Fusarium oxysporum، بكتريا حامض اللاكتيك، الجزيئات النانوية، Weissella cibariaالملخص
من المتوقع أن يتجاوز عبء السرطان العالمي المتوقع 20 مليون حالة سرطان جديدة بحلول عام 2025. على الرغم من التقدم الأخير في علاج الأورام ، لا يزال علاج السرطان الناجح يمثل تحديًا. يوفر مجال تكنولوجيا النانو الناشئ فرصًا رائعة لتشخيص السرطان ، والتصوير ، وعلاج السرطان. يعتبر التخليق الحيوي لجسيمات الفضة النانوية عن طريق الفطريات طريقة نظيفة وغير سامة بيئيًا مقارنة بالطرق الفيزيائية والكيميائية الأخرىالغرض من هذه الدراسة هو تحديد التأثير التآزري للجسيمات النانوية المركبة من Fusarium oxysporum مع Weisella cibaria ضد خلايا سرطان عنق الرحمأجريت هذه الدراسة من عام 2022 إلى مارس 2023 في معمل ميكروبيولوجيا الغذاء في قسم الأحياء / كلية العلوم / الجامعة المستنصرية. تم عزل بكتيريا حمض اللاكتيك (LAB) من مصادر الغذاء (الشلغم ، اللهانه ، الشوندر والقرنابيط) ، بعد خطوات متسلسلة من كلوريد الصوديوم المعالج ، ثم تم تربيتها في مرق MRS (Man-Rogosa-Sharpe) ، وتم فحصها تحت المجهر. تم الكشف عن النشاط المضاد للبكتيريا في المواد الطافية الخالية من الخلايا (CFS) التي تنتجها هذه العزلات لاختيار الأفضل وتشخيصها عن طريق تسلسل تفاعل البوليميراز المتسلسل والحمض النووي. تم الحصول على الجسيمات النانوية (AgNo3) التي تم إنتاجها من Fusarium oxysporum بالتخليق الحيوي من مختبر الدراسات العليا للفطريات ، وتم إخضاع هذه الفطريات لاختبار السمية. تمت دراسة تأثير التآزر للجسيمات النانوية المختارة من LAB و Fusarium oxysporum ضد خلايا سرطان عنق الرحم.
أظهرت هذه الدراسة أن جميع مصادر الغذاء كانت غنية بـ LAB وأن أفضل نشاط مضاد للجراثيم كان مصدر اللفت ووفقًا للتشخيص الجزيئي كان Weisella cibaria والذي سجل في NCBI كـ (MG7865551). أظهر التأثير التآزري لـ Weisella cibaria والجسيمات النانوية انخفاض معدل بقاء خط السرطان بعد 72 ساعة من التعرض لهذا التأثير.
Received 15/10/2022
Revised 04/08/2023
Accepted 06/08/2023
Published Online First 25/12/2023
المراجع
Torre LA , Islami F , Siegel RL , Ward EM , Jemal A. Global cancer in women: burden and trends global cancer in women: burden and trends .Cancer Epidemiol Biomarkers Prev . Apr 2017; 26(4): 444-457. https://doi.org/10.1158/1055-9965.EPI-16-0858. Epub 2017 Feb 21.
Ahmed AA , Hamzah H , Maaroof M. Analyzing formation of silver nanoparticles from the filamentous fungus Fusarium oxysporum and their antimicrobial activity. Turkish J Biol. Feb 2018; 42(1): 54-62. https://doi.org/10.3906/biy-1710-2. eCollection 2018.
Salazar L, López M, Grijalva M, Castillo L, Maldonado A. Biological effect of organically coated Grias neuberthii and Persea americana silver nanoparticles on HeLa and MCF-7 cancer cell lines. J Nanotech Aug 2018; 26(4): 444-457 . https://doi.org/10.1155/2018/9689131.
Shahzad A, Saeed H, Iqtedar M, Hussain SZ, Kaleem A, Abdullah R, Sharif S, Naz S, Saleem F, Aihetasham A, Chaudhary A. Size-controlled production of silver nanoparticles by Aspergillus fumigatus BTCB10: likely antibacterial and cytotoxic effects. J Nanomat. Feb 2019; 1-14. Article ID 5168698 . https://doi.org/10.1155/2019/5168698.
Korbekandi H , Ashari Z , Iravani S and Abbasi S. Optimization of biological synthesis of silver nanoparticles using Fusarium oxysporum. Iran J Pharma Res. Jul 2013; (12)3: 289-98.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813263
Wang Q, Li S, Zhao F, Dai H, Bao L, Ding R. Chemical constitutes from endophytic fungus Fusarium oxysporum. Fitoterapia. 2011; 82(5): 777-81. https://pubmed.ncbi.nlm.nih.gov/21497643/.
Lee KW, Park JY, Jeong HR, Heo HJ, Han NS, Kim JH. Probiotic properties of Weissella strains isolated from human feces. Anaerobe. Dec 2011; 18(1): 96-102
https://www.sciencedirect.com/science/article/abs/pii/S1075996411002368?via%3Dihub
Kang MS , Yen JE , Oh J , Shin B, Kim J. Complete Genome sequence of Weisella cibaria strains CMU, CMS1, CMS2 and CMS3 isolated from infant saliva is South Korea. American Society for Microbiology. Genome Announc .Oct 2017; 5(40): e01103-17. https://journals.asm.org/doi/10.1128/genomeA.01103-17.
Ojekunle O, Banwo K, Sanni AI. In vitro and in vivo evaluation of Weissella cibaria and Lactobacillus plantarum for their protective effect against cadmium and lead toxicities. Lett Appl Microbiol. May 2017; 64(5): 379-385. https://pubmed.ncbi.nlm.nih.gov/28276067/.
Björkroth KJ, Schillinger U, Geisen R, Weiss N, Hoste B, Holzapfel WH, Korkeala HJ, Vandamme P. Taxonomic study of Weissella confusa and description of Weissella cibaria sp. nov., detected in food and clinical samples. Int J Sys Evol Microbiol. 2002 Jan; 52(1): 141-8. https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-52-1-141
Mohsen YM, Shawket DS, Abd-AlSattar D. Novel Probiotic Bifidobacterium overcomes synergistic effect of three natural biotic OMNI Drug and antibiotic against some UTI pathogens. Int J Sci Nature. Jan 2013; 4(3): 456-462.
Askoul I , Gorrah S, Al-Amir L. Isolation and characterization of lactobacilli bacteria from some Syrian foods and detection the effectiveness of antipathogenic bacterial growth, Damascus J Agricul Scien . Feb 2014 ; 30(1): 227-237. https://shamra-academia.com/en/show/591b123434da2.
Holzapfel WH , Wood BJB. Lactic Acid Bacteria , Biodiversity and Taxonomy. John Wiley and Sons Ltd. UK. Apr 2014.[cited 2022 Nov2] .85p. https://doi.org/10.1002/9781118655252.ch1.
Abbas HH , Abd-Mohammed SA , Shawkat DS , Baker YM. Effect of Lactobacillus sp. Crude Bacteriocin (CB) and Cell-Free Supernatant (CFS) against E. coli growth and adherence on vaginal epithelial cell surface. Inter J Advan Res. Jan 2016; 1(1): 614-620. https://www.journalijar.com/uploads/705_IJAR-8328.pdf
Jasim EI, Shawket DS, Mohsin YMB, Mohammed BB, Ibraheem D A, Hussein MA. Comparative study between the effect of the Lactic Acid Bacteria and non against some pathogenic bacteria . Inter J Advan Biol Res. Mar 2017; 7(4): 656-660. https://www.researchgate.net/publication/328772759
Rahi GK , Ajah HA. Antagonistic activity of silver nanoparticles synthesis by Fusarium oxysporum against Candida spp. Pak J Biotechnol. June 2018; 15(2): 347-356. https://pjbt.org/index.php/pjbt/article/view/403.
Elavarasi V, Pugazhendhi A, Poornima TK, Valsala H , Thamaraiselvi K. Screening and characterization of Weissella cibaria isolated from food source for probiotic properties . Int J Comp Appl. Jan 2014; 1: 29-32. https://www.researchgate.net/publication/304245545_Screening_and_Characterization_of_Weissella_cibaria_Isolated_from_Food_Source_for_Probiotic_Properties.
Kamboj K, Vasquez A, Balada-Llasat JM. Identification and significance of Weissella species infections. Front Microbiol. 2015 Oct 31; 6: 1204.https://doi.org/10.3389/fmicb.2015.01204.
Mohsin YMB, Hasan AM. Dari WA. Natural product of Lactococcus overcome nosocomial infection in some of Baghdad hospitals in Iraq. Baghdad Sci J. Mar 2020; 1791: 227-234. https://doi.org/10.21123/bsj.2020.17.1(Suppl.).0227
Muhsin YMB , Majeed HZ. , Shawkat, D.S. CFS and crude bacteriocin of Lactococcus against growth and biofilm formation of some pathogenic bacteria. Int J Curr Micro App Sci.
Abdulhadi AS, Shafiq SA Mohsen YMB. Cytotoxic activity of partial purified proteins Saccharomyces and Lactic acid bacteria on HT-29 cell line. Ann Romanian Soc Cell Biol. June 2021; 25(7): 1670-1679.http://www.annalsofrscb.ro/index.php/journal/article/view/10597/7603.
Penido FC, de Oliveira Goulart C, Galvão YC, Teixeira CV, de Oliveira RB, Borelli BM, et al. Antagonistic lactic acid bacteria in association with Saccharomyces cerevisiae as starter cultures for standardization of sour cassava starch production. J Food Sci Technol . 2019 Sep 1; 56(9): 3969-79. https://doi.org/10.1007/s13197-019-03864-w.
Ali EN, Kadhem AA, Majeed HZ Mohsin YMB. Immunomodulatory effects of Pediococuus acidilactici on BALB/C mice immunosuppressed by cyclophosphamide. Res J Pharma Tech. Nov 2022; 15(12): 605-610. https://doi.org/10.52711/0974-360X.2022.00099.
Ahmed S, Singh S, Singh V, Roberts KD, Zaidi A, Rodriguez-Palacios A. The Weissella genus: Clinically treatable bacteria with antimicrobial/probiotic effects on inflammation and cancer. Microorganisms. 2022 Dec 7; 10(12):2427.https://doi.org/10.3390/microorganisms10122427
Shatti ZO, Mohammed BB Mohsin YMB .Four screening methods to determine Pediococcus acidilactici efficacy against two biofilm producing pathogenic bacteria., Ind J Publ Health Res. Apr 2020; 10(10): 3810-3814
Mohsin YMB, Majeed HZ Mohammed BB. Lactic acid bacteria biosurfactant role that isolated from human breast milk in inhabit eyes pathogenic bacteria. Ibn-Al-Haitham J Pure. Appl. Sep 2018; 31(2): 31-40. https://doi.org/10.30526/31.2.1959.
Mostafa F. Biosynthesis of silver nanoparticles by pathogenic and nonpathogenic strains of Fusarium oxysporum . sp. lycopersici. Egypt J Bot. Aug 2017; 57(2) : 345-350. https://ejbo.journals.ekb.eg/article_3615.html.
Korbekandi H, Ashari Z, Iravani S, Abbasi S. Optimization of biological synthesis of silver nanoparticles using Fusarium oxysporum. Iran J Pharma Res .Summer 2013; 12(3): 289 -298. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813263/.
Barik BP , Tayung K, Jagader P , Dutta SK. Phylogenetic Placement of an Endophytic Fungus Fusarium oxysporum Isolated from Acorus calamus Rhizomes with Antimicrobial Activity. Eur J Bio Sci. Jan- March 2010; 2(1): 8-16. https://www.researchgate.net/publication/228471582.
Ishida K , Cipriano TF , Rocha GM , Weissmuller G, Gomes F, Miranda K, et al. Silver nanoparticles production by the fungus Fusarium oxysporum: nanoparticle characterization and analysis of antifungal activity against pathogenic yeasts. Mem Inst Oswaldo Cruz, Rio de Janeiro , Apr 2014; 109(2): 220-8. https://doi.org/10.1590/0074- 0276130269.
Park JH , Ahn HJ , Kim SG, Chung CH. Dextran-like exopolysaccharide-producing Leuconstoc and Weissella from kimchi and its gradient. Food Sci Biotechnol. Aug 2013; 22: 1047- 1053. https://link.springer.com/article/10.1007/s10068-013-0182-x
Kwak S, Co Y, Noh G, Om A. Cancer preventive potential of kimchi lactic acid bacteria (W. cibaria, L. plantarum). J Cancer Prev. Dec 2014; 19 (4): 253-258. https://doi.org/10.15430/JCP.2014.19.4.253.
Husseiny SM , Salah TA , Anter H A. Biosynthesis of size controlled silver nanoparticles by Fusarium oxysporum, their antibacterial and antitumor activities .J Basic Appl Sci. Sep 2015; 4: 225-231. https://doi.org/10.1016/j.bjbas.2015.07.004
Jalgaonwala R ,Mahajan RT. Production of anti-cancer enzyme asparaginase from endophytic Eurotium sp. isolated from rhizomes of Curcuma longa. Eur J Exp Biol . Oct 2014; 4(3): 36-43. https://www.primescholars.com/articles/production-of-anticancer-enzyme-asparaginase-from-endophyticeurotium-sp-isolated-from-rhizomes-of-curcuma-longa-91486.html.
Yadav N, Surkar S. Production of asparaginase by Fusarium oxysporum using submerged fermentation. Int J Pharma Sci Invent. June 2014; 3(6): 32-40. http://www.ijpsi.org/Papers/Vol3(6)/G0361032040.pdf
Chow YY, Ting ASY. Endophytic L-asparaginase producing fungi from plants associated with anticancer properties. J Adv Res. Nov 2015; 6(6): 869-867. https://doi.org/10.1016/j.jare.2014.07.005.
Auda IG, Mohsin YM, Jasim EI ,Sharan Z. Ouda JG. Partial sequencing ISI2I6V transpsase gene of Staphylococcus aureus isolated from samples , Al-Kindy Coll Med J. Jan 2018; 14(2): 24- 26. https://doi.org/10.47723/kcmj.v14i2.47
Abdel-Rahman TM , Khalil NM, El-Ghany MN , Yosef E. Purification , characterization and medicinal application of tyrosinase extracted from Saccharomyces cerevisiae . J Innov. Pharm Biol Sci. Jan 2019; 6(1): 1-11.
Al-Kassie G, Al-jumaa Y, Jamel Y. Effect of probiotic (Aspergillus niger) and prebiotic (Taraxacum officinale)on blood picture and biochemical properties of broiler chicks. Int J Poult Sci. July 2018; 7.(12): 1182-1184. https://doi.org/10.3923/ijps.2008.1182.1184
Al-Shammari AM, Al-Saadi H, Al-Shammari SM, Jabir MS. Galangin enhances gold nanoparticles as anti-tumor agents against ovarian cancer cells. AIP Conf Proce .Mar 25 2020; 2213(1): 020206-1–020206-7.AIP Publishing. https://doi.org/10.1063/5.0000162.
Haryani Y, Halid NA, Guat GS, Nor-Khaizura MA, Hatta A, Sabri S, et al. Characterization, molecular identification, and antimicrobial activity of lactic acid bacteria isolated from selected fermented foods and beverages in Malaysia. FEMS Microbiol. Lett. 2023; 370: fnad023. https://doi.org/10.1093/femsle/fnad023.
Haryani Y, Halid NA, Guat GS, Nor-Khaizura MA, Hatta A, Sabri S, et al. Characterization, molecular identification, and antimicrobial activity of lactic acid bacteria isolated from selected fermented foods and beverages in Malaysia. FEMS Microbiol. Lett. 2023; 370: fnad023. https://doi.org/10.1093/femsle/fnad023.
Musah SS, Owuna JE, Makut MD, Adamu BB, GI I, Izebe K, et al. Antibacterial activity of lactic acid bacteria isolated from Etsako Osuemegbe rice. AROC Food Nutr. 2023; 2(1): 01-05. https://doi.org/10.53858/arocfn02010105.
Bartkiene E, Lde V Ruzauskas M. Lactic acid bacteria isolation from spontaneous sourdough and their characterization including antimicrobial and antifungal properties evaluation. Microorganisms. Dec 2020; 8(1): 64 https://doi.org/10.3390/microorganisms8010064.
Walia S, Kamal R ,Kanwar SS. Chemoprevention by Probiotic during 1,2-Dimethyl hydrazine induced colon carcinogenesis. Dig Dis Sci Apr 2018: 63(4): 900-909. https://doi.org/10.1007/s10620- 018-4949-z.
Kiczorowski P , Kiczorowski B, Mieczan A. Effect of fermentation of chosen vegetables on the nutrient , mineral and biocomponent profile in human and animal nutrition. Sci Rep. Aug 2022; 12: 13422. https://doi.org/10.1038/s41598-022-17782-z
Junnarkar M ,Pawar S, Gaikwad S. Nawani N. Probiotic Potential of Lactic Acid Bacteria from fresh vegetables : Application in food preservation .Ind J Exp Biol. Oct 2019; 57 : 825-838. https://www.divaportal.org/smash/get/diva2:1375657/FULLTEXT02.pdf.
Poore J. Nemecek T. Reducing foods environmental impact through producers and consumers. Science. Jun 2018; 360 : 987-992 . https://doi.org/10.1126/science.aaq0216
Joch M, Kudrana V. Hucko B. Effect of Geraniol and Camphene on in vitro rumen fermentation and methane production . Sci Agric Bohem. Jan 2017; 48: 63-69. https://doi.10.1515/sab-2017-0012.
Grenda T, Grenda A, Domaradzki P, Kwiatek K. Probiotic potential of Clostridium spp. advantages and Doubts .Curr Issues Mol .Biol .Jul 2022; 44 (7): 3118-3130. https://doi.org/10.3390/cimb44070215.
Manik M, Kaban J, Silalahi J Gimting M. Lactic Acid Bacteria (LAB) with probiotic potential from Dengka Naniura. Baghdad Sci J .Mar 2021; 18 (1): 35-40.https://doi.org/10.21123/bsj.2021.18.1.0035.
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