مكافحة الذبول الفيوزارمي على الفلفل باستعمال عامل المكافحة الاحيائية Trichoderma harzianum والحديد المخلبي Fe-EDDHA

محتوى المقالة الرئيسي

Ali Nasser Ali Al-aamel
https://orcid.org/0009-0006-4660-4741
Bushra Subair Abdulsada Al-maliky
https://orcid.org/0000-0001-9103-7113

الملخص

الفلفل الحلو من المحاصيل الاقتصادية المهمة، يعد مرض الذبول المتسبب عن الفطر Fusarium oxysporum f. sp. capsici من الامراض المهمة التي تؤثر على الفلفل. تم الحصول على اربع عزلات منF. oxysporum   (Fo3، Fo6، Fo7 و Fo8) من نباتات فلفل مصابة جمعت من حقول مختلفة في محافظة بغداد. اظهرت العزلة Fo6 امراضية عالية على بذور الفلفل، تم اختبار Trichoderma harzianum (Th) ضد  F. oxysporum f. sp. capsici في المختبر واظهر نسبة تثبيط عالية ضد العزلة Fo6، الحديد المخلبي بتركيز 0.5٪ قلل من النمو الشعاعي للعزلة Fo6 بينما لم يؤثر على نمو Th. في تجربة الاصص، اظهرت المعاملة Fo6+Th+Fe خفضاً معنوياً في نسبة وشدة الاصابة بمرض الذبول وتعفن الجذور قياساً مع المقارنة. كما حققت المعاملة Th+Fe زيادة عالية المعنوية في الوزن الطري والجاف للمجموعين الجذري والخضري اذ بلغت 63.36، 130.56، 4.55 و 10.26غم على الترتيب قياساً بالمقارنة (من دون الممرض) التي سجلت 11.10، 54.83، 1.30 و 3.70غم على الترتيب. واوضحت نتائج تجربة البيت البلاستيكي ان المعاملة Fo6+Th+Fe خفضت نسبة وشدة الاصابة بالعزلة Fo6 اذ بلغت 3.33 و 1.67% على الترتيب واعطت المعاملة Th+Fe ارتفاع في محتوى الكلوروفيل الكلي الذي بلغ 104 سباد قياساً بالمقارنة التي سجلت 70.4 سباد.  

تفاصيل المقالة

كيفية الاقتباس
1.
مكافحة الذبول الفيوزارمي على الفلفل باستعمال عامل المكافحة الاحيائية Trichoderma harzianum والحديد المخلبي Fe-EDDHA. Baghdad Sci.J [انترنت]. 1 ديسمبر، 2023 [وثق 19 مايو، 2024];20(6). موجود في: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/8037
القسم
article

كيفية الاقتباس

1.
مكافحة الذبول الفيوزارمي على الفلفل باستعمال عامل المكافحة الاحيائية Trichoderma harzianum والحديد المخلبي Fe-EDDHA. Baghdad Sci.J [انترنت]. 1 ديسمبر، 2023 [وثق 19 مايو، 2024];20(6). موجود في: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/8037

المراجع

Parisi M, Alioto D, Tripodi P. Overview of biotic stresses in pepper (Capsicum spp.): Sources of genetic resistance, molecular breeding and genomics. Int J Mol Med Sci. 2020; 21(7): 1-39. https://doi.org/10.3390/ijms21072587.

Karimi B, Maron PA, Chemidlin-Prevost Boure N, Bernard N, Gilbert D, Ranjard L. Microbial diversity and ecological networks as indicators of environmental quality. Environ Chem Lett. 2017; 15(2): 265-81. https://doi.org/10.1007/s10311-017-0614-6.

Kumar M, Vipul K, Meenakshi R, Seweta S. Effect of volatile and non-volatile compounds of Trichoderma spp. against Fusarium isolates causing chickpea wilt in Punjab. Plant Archiv. 2019; 19(1): 159-162.

Nwankiti AO, Gwa VI. Evaluation of antagonistic effect of Trichoderma harzianum against Fusarium oxysporum causal agent of white yam (Dioscorearotundata poir) tuber rot. Trends Tech. Sci. Res. 2018; 1(1): 12-18. https://doi.org/1019081/TTSR.2018.01.555554.

Mosleh MF, Rasool IA, Role of spraying boron and sugar alcohols on growth, yield and seeds production of pepper. Iraqi J. Agric. Sci. 2019; 50(2): 646-652. https://doi.org/10.36103/ijas.v2i50.664.

Shrestha J, Kandel M, Subedi S, Shah KK. Role of nutrients in rice (Oryza sativa L.): A review. Agrica. 2020; 9(1): 53-62. https://doi.org/10.5958/2394-448X.2020.00008.5.

Al-Dulaimi NH, Al-Jumaili MA. Response green beans to spray some micronutrients and adition organic fertilizer. Iraqi J. Agric. Sci. 2017; 48(2): 447-455. https://doi.org/10.36103/ijas.v48i2.407.

Aznar A, Chen NW, Thomine S, Dellagi A. Immunity to plant pathogens and iron homeostasis. Plant Sci. 2015; 240: 90-97. https://doi.org/10.5958/2394-448X.2020.00008.510.1016/j.plant sci.2015.08.022.

Pylak M, Oszust K, Frąc, M. Review report on the role of bioproducts, biopreparations, biostimulants and microbial inoculants in organic production of fruit. Rev Environ Sci Biotechnol. 2019; 18(3): 597-616. https://doi.org/10.1007/s11157-019-09500-5.

Ramirez-Pimentel JG, Aguirre-Mancilla CL, Raya-Pérez JC, Acosta-García G, Iturriaga G. Trichoderma harzianum mutants enhance antagonism against phytopathogenic fungi, phosphorus assimilation and drought tolerance in Jalapeño pepper plants. Chil J Agric Res. 2021; 81(3): 270-280. http://dx.doi.org/10.4067/S0718-58392021000300270.

Noman E, Al-Gheethi AA, Rahman NK, Talip B, Mohamed R, Kadir OA. Single spore isolation as a simple and efficient technique to obtain fungal pure culture. IOP Conf. Ser.: Earth Environ. Sci. 2018; 140(1): 2-6. https://doi.org/10.5958/2394-448X.2020.00008.510.1088/1755-1315/140/1/012055.

Velarde Felix S, Ortega Murrieta PF, Fierros Leyva GA, Padilla Valenzuela I, Gutierres Pérez E, Rodriguez Cota FG, et al. Identificacion molecular y biologica de las razas 0 y 5 de Fusarium oxysporum Schlechtend.: Fr f. sp. ciceris (Padwick) Matuo & K. Sato del garbanzo en el noroeste de Mexico. Rev Mex De Cienc Agric, 2015; 6(4): 735-748. https://doi.org/10.29312/remexca.v6i4.615.

White TJ, Bruns T, Lee SJ, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications. 1990; 18(1): 315-22. https://doi.org/10.5958/2394-448X.2020.00008.510.1016/B978-0-12-372180-8.50042-1.

Bolkan HA, Butler EE. Studies on heterokaryosis and virulence of Rhizoctonia solani. Phytopathology. 1974; 64(5): 13-522, DOI: 10.1094/Phyto-64-513.

Bell JM, Shires A. Composition and digestibility by pigs of hull fractions from rapeseed cultivars with yellow or brown seed coats. Can J Anim Sci. 1982; 62(2): 557-565. https://doi.org/10.4141/cjas82-065.

Dong X, Wang M, Ling N, Shen Q, Guo S. Effects of iron and boron combinations on the suppression of Fusarium wilt in banana. Sci Rep. 2016; 6(1): 1-11. https://doi.org/10.1038/srep38944.

Yaseen RA, Ibrahim MA, Mustafa MA. The effect of Schanginia aegyptica and Urtica dioica powder on the growth of Trigonella foenum seedlings in laboratory sterilized soil. HIV Nursing. 2022; 22(2): 243-247. https://doi.org/10.5958/2394-448X.2020.00008.510.31838/hiv22.02.50.

Sun R, Zhang XX, Guo X, Wang D, Chu H. Bacterial diversity in soils subjected to long-term chemical fertilization can be more stably maintained with the addition of livestock manure than wheat straw. Soil Biol Biochem. 2015; 88: 9-18. https://doi.org/10.1016/j.soilbio.2015.05.007.

Al-Kaisy WA, Mahmood RW, Hameid AS. Effect of proline and aspirin on seed germination and seedling growth of Lycopersicon esculentum and surface growth of Fusarium oxysporum. Baghdad Sci. J. 2014; 11(2): 813-818. https://doi.org/10.21123/bsj.2014.11.2.813-818.

Robles-Carrion A, Leiva-Mora M, Herrera-Isla I, Sánchez-Rodríguez A, Torres-Gutiérrez R. Morphological and molecular identification of Fusarium species associated with vascular wilt of babaco (Vasconcellea heilbornii var. pentagona Badillo). Rev Prot Veg. 2016; 31(3): 184-193. https://doi.org/10.5958/2394-448X.2020.00008.510.21161/mjm.200938.

Hateet RR. Evaluation of bioactivity against some pathogenic bacteria and oxidation for fungal secondary metabolites of Fusarium solani isolated from soil. Baghdad Sci. J. 2017; 14(3): 497-502. https://doi.org/10.21123/bsj.2017.14.3.0497.

Liu S, Li J, Zhang Y, Liu N, Viljoen A, Mostert D, et al. Fusaric acid instigates the invasion of banana by Fusarium oxysporum f. sp. cubense TR 4’. New Phytol. 2020; 225(2): 913-929. https://doi.org/10.1111/nph.16193.

Abdel-Ghany TM, Bakri MM. Effectiveness of a Biological Agent (Trichoderma harzianum and its culture filtrate) and a Fungicide (methyl benzimacold-2-ylcarbamate) on the Tomato Rotting Activity (growth, cellulolytic, and pectinolytic activities) of Alternaria solani. Bioresources. 2019; 14(1): 1591-602. https://doi.org/10.5958/2394-448X.2020.00008.510.15376/biores.14.1.1591-1602.

Anjum N, Shahid AA, Iftikhar S, Mubeen M, Ahmad MH, Jamil Y, Rehan MK, Aziz A, Iqbal S, Abbas A. Evaluations of Trichoderma isolates for biological control of Fusarium wilt of Chili. Plant Cell Biotechnol Mol Biol. 2020; 30(21): 59-60.

Lei Z, Qun L, Zhang YQ, Cuiqy, Liang YC. Effect of acid phosphatase produced by Trichoderma asperellum Q1 on growth of Arabidopsis under salt stress. J Integr Agric. 2017; 16(6): 1341-6. https://doi.org/10.1016/S2095-3119(16)61490-9.

Segarra G, Casanova E, Avilés M, Trillas I. Trichoderma asperellum strain T34 controls Fusarium wilt disease in tomato plants in soilless culture through competition for iron. Microb Ecol 2010; 59(1): 141-149. https://doi.org/10.5958/2394-448X.2020.00008.510.1007/s00248-009-9545-5.

Wiebe MG. Siderophore production by Fusarium venenatum A3/5. Biochem Soc Trans. 2002; 30 (4): 696-698. https://doi.org/10.1042/bst0300696.

Li YT, Hwang SG, Huang YM, Huang CH. Effects of Trichoderma asperellum on nutrient uptake and Fusarium wilt of tomato. Crop Protection. 2018; 110: 275-82. https://doi.org/10.1016/j.cropro.2017.03.021.

Duc NH, Mayer Z, Pék Z, Helyes L, Posta K. Combined inoculation of arbuscular mycorrhizal fungi, Pseudomonas fluorescens and Trichoderma spp. for enhancing defense enzymes and yield of three pepper cultivars. Appl Ecol Environ Res. 2017; 15(3) 1815-1829. https://doi.org/10.5958/2394-448X.2020.00008.510.15666/aeer/1503_18151829.

Karapetyan S, Dong X. Redox and the circadian clock in plant immunity: A balancing act. Free Radic Biol Med. 2018; 11: 56-61. https://doi.org/10.5958/2394-448X.2020.00008.5 10.1016/j.freeradbiomed.2017.12.024.

AL-Surhanee AA, ALmashari RM, ALkhluif SHA. The effect of Trichoderma harizanum fungus usage as bio-fertilizer on tomato seedling growth. Iraqi J. Agric. Sci. 2017; 48(4): 1115-1121. https://doi.org/10.36103/ijas.v48i4.369.

Asari S, Tarkowská D, Rolčík J, Novák O, Palmero DV, Bejai S, Meijer J. Analysis of plant growth-promoting properties of Bacillus amyloliquefaciens UCMB5113 using Arabidopsis thaliana as host plant. Planta. 2017; 245(1): 15-30. https://doi.org/10.5958/2394-448X.2020.00008.510.1007/s00425-016-2580-9. Epub 2016 Aug 19.

Basu A, Prasad P, Das SN, Kalam S, Sayyed, RZ, Reddy MS, ElEnshasy H.. Plant growth promoting rhizobacteria (PGPR) as green bioinoculants: recent developments, constraints, and prospects. Sustainability. 2021; 13(3): 1-20. https://doi.org/10.5958/2394-448X.2020.00008.510.3390/su13031140.

Dar GH, Mir GH, Rashid H, Dar WA, Majeed M. Evaluation of microbial antagonists for the management of wilt/root rot and damping-off diseases in chilli (Capsicum annuum). Vegetos. 2015; 28(4): 102-110 https://doi.org/10.5958/2394-448X.2020.00008.510.5958/2229-4473.2015.00091.9.

Koijam K, Sinha B. Antagonistic potential and molecular characterization of Trichoderma spp. against Rhizoctonia solani infecting ghost pepper in Manipur. Int. J. Curr. Microbiol. App. Sci. 2018; 7(2): 2085-93. https://doi.org/10.20546/ijcmas.2018.702.248.

Wang X, Cai A, Wen X, Jing D, Qi H, Yuan H. Graphene oxide-Fe3O4 nanocomposites as high-performance antifungal agents against Plasmopara viticola. Sci China Mater. 2017; 60(3): 258-68. https://doi.org/10.1007/s40843-016-9005-9.

Zhao RZ, Jiang S, Zhang L, Yu ZB. Mitochondrial electron transport chain, ROS generation and uncoupling. Int J Mol Med. 2019; 44(1): 3-15. https://doi.org/10.5958/2394-448X.2020.00008.510.3892/ijmm.2019.4188.

Roosta HR, Estaji A, Niknam F. Effect of iron, zinc and manganese shortage-induced change on photosynthetic pigments, some osmoregulators and chlorophyll fluorescence parameters in lettuce. Photosynthetica. 2018; 56(2): 606-15. https://doi.org/10.1007/s11099-017-0696-1.

Liu C, Ye Y, Liu J, Pu Y, Wu C. Iron biofortification of crop food by symbiosis with beneficial microorganisms. J Plant Nutr. 2021; 44(18): 2793-2810. https://doi.org/10.1080/01904167.2021.1927089.

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