التقدير اللوني للسيتوكوبال (فيتامين ب 12) بطريقة غير مباشرة باستخدام الأيونات الحديديوز و 1،10 الفينانثرولين
DOI:
https://doi.org/10.21123/bsj.2024.9100الكلمات المفتاحية:
Colorimetric method, CyanoCobalamin, Ferrous Sulfate ,1,10-phenanthroline,Vitamin B12.الملخص
تم تقدير CyanoCobalamin (فيتامين ب 12) باستخدام طريقة لونية بسيطة وموثوقة ومعقولة التكلفة. وفقًا لهذه الطريقة ، يتم استخدام زيادة من كبريتات الحديدوز (FeSO4.7H2O ، أو FS) لاختزال فيتامين B12 ، ويتفاعل FS الزائد مع الكاشف 1،10-فينانثرولين لتكوين مركب أحمر قابل للذوبان في الماء. كان الطول الموجي الأعظم للطيف لهذا المركب هو 510 نانومتر وكان مدى خطية التركيز (5-100 ميكروجرام / مل) وكانت قيم %RSD خلال اليوم الواحد 0.107-0.259% و 1.085-0.1378٪ بين يوم و اخر، في حين تراوحت قيم %Rec من 95 إلى 100.833%. كانت قيم LOD و LOQ على التوالي 0.016 ميكروغرام / مل و 0.053 ميكروغرام / مل. تم استخدام الطريقة لتقدير فيتامين ب 12 في المستحضرات الطبية بنجاح
Received 20/05/2023
Revised 05/11/2023
Accepted 07/11/2023
Published Online First 20/07/2024
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