Colorimetric Determination of Cyanocobalamin (VitaminB12) by indirect method with Ferrous ion and 1,10 phenanthroline
DOI:
https://doi.org/10.21123/bsj.2024.9100Keywords:
طريقة لونية ، سيانوكوبال امين ، كبريتات الحديدوز، 1،10- فينانترولين و فيتامين ب12.Abstract
CyanoCobalamin (Vitamin B12) was determined using a simple, reliable, and affordable colorimetric method. According to this method, excess Ferrous sulfate (FeSO4.7H2O, or FS) is used to reduce vitamin B12, and the excess FS reacts with the reagent 1,10-phenanthroline to produce a soluble red color complex. The greatest wavelength of the spectrum of this complex was 510 nm. The concentration linearity range was 5-100 µg/mL. The RSD% values were 0.107-0.259% for intraday and 1.085-0.1378% for interday, while the Rec% values ranged from 95 to 100.833%. LOD and LOQ values were respectively 0.016 µg/mL and 0.053 µg/mL. The method was used to determine the presence of vitamin B12 in medicinal preparations with success.
Received 20/05/2023
Revised 05/11/2023
Accepted 07/11/2023
Published Online First 20/07/2024
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