Evaluation of Antioxidants, Antibacterial and Antidiabetic Activities of Aqua-alcoholic Marjoram Extract
DOI:
https://doi.org/10.21123/bsj.2024.9328Keywords:
Antibacterial activity, Antidiabetic activity, Antifungal activity, Antioxidant activity, Marjoram extractAbstract
Plant extracts possess special significance towards human health and vital activities for a wide range of diseases. The current study aims at verifying the presence of active ingredients in marjoram leaves extract, and determining antioxidants, antibacterial and antifungal activities, LD50 and its antidiabetic activity. The active ingredients were qualitatively analyzed by specific test methods. Antioxidant, LD50, antibacterial and antifungal activities were measured by their standard method. The antidiabetic activity was determined by assessed hyperglycemia, hyperlipidemia and liver functions by spectrophotometric methods. The results confirmed the presence of several active components including flavonoids, alkaloids, terpenoids, tannins, and saponins. Also, the results of antioxidants activity showed that marjoram leaves extract possesses an antioxidant activity similar to ascorbic acid. LD50 results revealed that the allowed dose is 12.5 mg/ml. Biological activity results indicated that marjoram leaves extract possesses antibacterial and antifungal activities against wide range of pathogenic bacteria and fungi, but there is no effect of extract on E. coli and Staphylococcus aureus even at high concentrations. Marjoram extract revealed high antidiabetic activity when treated for two weeks 4mg of the extract daily. Also, lipid profile and liver functions, and testosterone levels were improved after treatment in the diabetic group related to the control group. It can be concluded that marjoram leaves extract has high antioxidant activity with high biological activity and high antidiabetic activity with a significant enhancement of fertility affected in diabetes and a high level of tolerated doses according to the LD50 value.
Received 10/09/2023
Revised 29/12/2023
Accepted 31/12/2023
Published Online First 20/01/2024,
References
Hamad SF, Salman ZO, Alwash BMJ. Assessment of Antioxidant and Cytotoxic activity of Essential Oil Extracted from Lavandola Angustifolia Callus Leaves. Iraqi J Agric Sci. 2021; 52(6): 1549-1554. https://doi.org/10.36103/ijas.v52i6.1496
Kareem AJ. Alwash BMJ. Enhancement phenolic content in callus taraxacum officinale plant by using phenyl alanine precursors. Biochem Cell Arch. 2022; 22 (1): 3119-3125. https://doi.org/10.51470/bca.2022.22.2.3545
Al-Azzawi MA, Saleh WR, Rashid FA, Alwash BMJ. Synthesis and Characterization of Nanoparticles Extracted from Catharanthus roseus Plant. Nano Hybrids and Composites. 2023; 38: 15-24. https://doi.org/10.4028/p-z7zy4l
Abdulhalem AG, Alwash BMJ, Ibrahim KM. Localization of Saponins Accumulation in Yucca Gloriosa Variegata in Intact Tissues and Their Tissue Cultures by Using Fluorescence Microscopy. Ann Romanian Soc Cell Biol. 021; 25(6): 8615-8626.
Alwash BMJ, Salman ZO, Hamad SF. Qualitative and Quantitative Evaluation of Active Constituents in Callus of Lavandula angustifolia plant in Vitro. Baghdad Sci J. 2020; 17 (2 Special Issue): 591-598. http://dx.doi.org/10.21123/bsj.2020.17.2(SI).0591
Abid Aun ZA, Habeeb HM, Jazaa LA. Morphological, Anatomical and Chemical Study of an Exotic Plant Jatropha Integerrima JACO.1763 (Euphorbiaceae) in Iraq. Bull Iraq Nat Hist Mus. 2022; 17 (1): 129-140. https://doi.org/10.26842/binhm.7.2022.17.1.0129
Mohammed S, Habeeb HM. Phytochemical and cytotoxic study in Quisqualis indica volatile oil cultivation in Iraq. Int J Health Sci. 2022; 6(S4): 8572– 8584. https://doi.org/10.53730/ijhs.v6nS4.10618
Hamid GS, Allawi AAD, Ghudhaib KK. Correlation of Pentosidine with Kidney Diseases in Iraqi Patients with Diabetic Nephropathy. Iraqi J Sci. 2021; 62(10): 3436-3442. https://doi.org/10.24996/ijs.2021.62.10.2
Ibrahim RK, Allawi AAD, Ghudhaib KK. Determining ACE-2 Level and Some Relevant Biochemical Parameters and Studying the effect of Gender in Iraqi Diabetic Patients with Glomeruli and Renal Tubules Fibrosis as Early Prediction Marker. Baghdad Sci J., 2023. https://doi.org/10.21123/bsj.2023.7633
Salman ZA, Ghudhaib KK, Fadhil R. Association of osteopontin and alkaline phosphatase in male patients with diabetes mellitus type 2 and periodontitis. Iran J War Public Health. 2022; 14(1): 105-109. https://doi.org/10.24996/ijs.2021.62.10.2
Ibrahim RK, Ghudhaib KK, Allawi AA. Early Prediction of Nephropathy in Iraqi Patients with Diabetes Type II by Evaluating of some Relevant Biochemical Factors. Baghdad Sci J. 2023 https://doi.org/10.21123/bsj.2023.8008
Bina F, Rahimi R. Sweet Marjoram: A Review of Ethnopharmacology, Photochemistry, and Biological Activities J Evid Based Complementary Altern Med. 2017; 22(1): 175–185. https://doi.org/10.1177/2156587216650793
Kozłowska M, Scibisz I, Przybył JL, Laudy AE, Majewska E, Tarnowska K, et al. Antioxidant and Antibacterial Activity of Extracts from Selected. Plant Mater Appl Sci. 2022; 12 (19): 9871-9891. https://doi.org/10.3390/app12199871
Ziarno M, Kozłowska M, Scibisz, I, Kowalczyk M, Pawelec S, Stochmal A, et al. The effect of selected herbal extracts on lactic acid bacteria activity. Appl Sci. 2021; 11: 3898. https://doi.org/10.3390/app11093898
Saifulazmi NF, Rohani ER, Harun S, Bunawan H, Hamezah HS, Muhammad NA, et al. A Review with Updated Perspectives on the Antiviral Potentials of Traditional Medicinal Plants and Their Prospects in Antiviral Therapy. Life. 2022; 12: 1287. https://doi.org/10.3390/life12081287
Guchu BM, Machocho AK, Mwihia SK, Ngugi MP. In Vitro Antioxidant Activities of Methanolic Extracts of Caesalpinia volkensii Harms., Vernonia lasiopus O. Hoffm., and Acacia hockii De Wild. J Evid Based Complementary Alternat Med. 2020; 2020: 1-10. https://doi.org/10.1155/2020/3586268
Kedir WM, Geletu AK, Weldegirum GS, Sima MF. Antioxidant activity of selected plants extract for palm oil stability via accelerated and deep-frying study. Heliyon. 2023; 9: e17980. https://doi.org/10.1016/j.heliyon.2023.e17980
Fernandes VC, Queiroz CR, Almeida ES, Melo CMT. Phenolic Content and Antioxidant Activity of Medicinal Plants Res Soc Dev. 2023; 12(3): 1-11. http://dx.doi.org/10.33448/rsd-v12i3.40475
Ali SA, Khalid ZM, Khan MR, Ashraf S, Ahmad N, Karami AM, et al. Evaluation of some Essential Traditional Medicinal Plants fort their Potential Free Scavenging and Antioxidant Properties. J King Saud Univ Sci. 2023; 35 (2): 1-13. https://doi.org/10.1016/j.jksus.2023.102562
Erfan AM, Marouf S. Cinnamon oil downregulates virulence genes of poultry respiratory bacterial agents and revealed significant bacterial inhibition: An in vitro perspective. Vet World. 2019; 12(11): 1707–1715. https://doi.org/10.14202/vetworld.2019.1707-1715
Hemeg HA, Moussa IM, Ibrahim S, Dawoud TM, Jwaher H. Alhaji JH, et al. antimicrobial effect of different herbal plant extracts against different microbial population. Saudi J Biol Sci. 2020; 27(12): 3221–3227. https://doi.org/10.1016/j.sjbs.2020.08.015
Muntean D, Vulpie S. Antioxidant and Antibacterial Activity of Plant Extracts. Antibiotic. 2023; 12(7): 1176-1177. https://doi.org/10.3390/antibiotics12071176
Stuper-Szablewska K, Szablewski T, Przybylska-Balcerek A, Szwajkowska-Michałek L, Michał Krzyżaniak M, Świerk D. Antimicrobial Activities Evaluation and Phytochemical Screening of Some Selected Plant Materials Used in Traditional Medicine. Molecules 2023; 28(1): 244-265. https://doi.org/10.3390%2Fmolecules28010244
Wasihun Y, Alekaw H, Ayenew KD. Antibacterial Activity and Phytochemical Components of Leaf Extract of Calpurnia aurea. Sci Rep. 2023; 13 (1): 9767- 9774. https://doi.org/10.1038/s41598-023-36837-3
Muhammad AA, Nisar Z, Shah SMA, Mustafa I, Nisar J, Asif R. Antibacterial Activity of Economically Important Medicinal Plants in Pakistan Against Different Bacterial Strains. Bioinform Biol Insights. 2023; 17: 1-6. https://doi.org/10.1177/11779322231189374
Kifle ZD, Abdelwuhab MB, Melak AD, Genet GM, Meseret T, Adugna M. Pharmacological Evaluation of Medicinal Plants with Antidiabetic Activities in Ethiopia: A review. Metab Open. 2022; 10 (13): 1-9. https://doi.org/10.1016/j.metop.2022.100174
Belayneh YM, Birru EM. Antidiabetic Activities of Hydromethanolic Leaf Extract of Calpurnia aurea (Ait.) Benth. Subspecies aurea (Fabaceae) in Mice. J Evid Based Complementary Altern Med. 2018; 2018: 1-9. https://doi.org/10.1155/2018/3509073
Akhtar MS, Rafullah M, Hossain1 MA, Ali M. Antidiabetic activity of Cichorium intybus L water extract against streptozotocin‑induced diabetic rats. J Umm Al-Qura Univer. Appl Sci. 2023(9): 565-571. https://doi.org/10.1007/s43994-023-00066-1
Cele ND, Mthimunye NE, Mkhwanazi OB, Nxumalo S, Tshabuse F, Pooe OJ et al. In Vitro Antidiabetic, Antioxidant, and Cytotoxic Evaluation of Honeybush Tea (Cyclopia genistoides) Extracts. J Food Bioch. 2023; 4: 1-11. https://doi.org/10.1155/2023/8774094
Magar AB, Shrestha D, Pakka S, Sharma KR. Phytochemistry, Biological, and Toxicity Study on Aqueous and Methanol Extracts of Chromolaena odorata. Sci World J. 2023; 2: 1-11. https://doi.org/10.1155/2023/6689271
Mujeeb F, Bajpai P, .Pathak N. Phytochemical evaluation, antimicrobial activity, and determination of bioactive components from leaves of aegle marmelos. Biomed Res Int. 2014: 1-11. https://doi.org/10.1155/2014/497606
Litchfield JT, Wilcoxon F. A simplified method of evaluating dose- effect experiments. J Pharmacol Exp Ther. 1949 Jun; 96(2): 99-113. https://github.com/JVAdams/LW1949
Ohwada K. Agar Plate Method for Detection and Enumeration of Alkylbenzenesulfonate-Degrading Microorganisms. Appl Micro. 1975 Jan; 29(1): 40-43. https://doi.org/10.1128/am.29.1.40-43.1975
Chaves N, Santiago A, Alías JC. Quantification of the antioxidant activity of plant extracts: Analysis of sensitivity and hierarchization based on the method used. Antioxidants. 2020; 9(1): 76-90. https://doi.org/10.3390/antiox9010076
Zlatić N, Jakovljević D, Stanković M. Temporal, plant part, and interpopulation variability of secondary metabolites and antioxidant activity of Inula helenium L. Plants. 2019; 8(6): 179-188. https://doi.org/10.3390%2Fplants8060179
Liang J, Huang X, Ma G. Antimicrobial activities and mechanisms of extract and components of herbs in East Asia RSC Adv. 2022; 12: 29197-29213. https://doi.org/10.1039/D2RA02389J
Hamilton MA, Russo RC, Robert V. Environ T. Trimmed Spearman-Karber method for estimating median lethal concentrations in toxicity bioassays. Environ Sci Technol. 1977; 11 (7):714–719. https://doi.org/10.1021/es60130a004
Wisnu M, Putra A, Fakhrudin N, Nurrochmad A, Wahyuono S. A Review of Medicinal Plants with Renoprotective Activity in Diabetic Nephropathy Animal. Life (Basel). 2023; 13(2): 560- 592. https://doi.org/10.3390/life13020560
Lee J, Noh S, Lim S, Kim B. Plant Extracts for Type 2 Diabetes: From Traditional Medicine to Modern Drug Discovery. Antioxidant. 2021; 10 (1): 81-121. https://doi.org/10.3390/antiox10010081
Espinoza -Hernández FA, Moreno-Vargas AD, Andrade-Cetto A. Diabetes-Related Mechanisms of Action Involved in the Therapeutic Effect of Croton Species: A Systematic Review. Plants. 2023; 12(10): 1-22 https://doi.org/10.3390/plants12102014
Ramchoun M, Khouya T, Harnafi H, Amrani S, Alem C, Benlyas M, et al . Effect of Aqueous Extract and Polyphenol Fraction Derived from Thymus atlanticus Leaves on Acute Hyperlipidemia in the Syrian Golden Hamsters. J Evid Based Complementary Alternat Med. 2020:1-9. https://doi.org/10.1155/2020/3282596
Đorđević MM, Tolić A, Rajić J, Mihailović M, Jovanović A, Uskoković AA. Centaurium erythraea methanol extract improves the functionality of diabetic liver and kidney by mitigating hyperglycemia-induced oxidative stress. J Funct Foods. 2022; 90: 104975. https://doi.org/10.1016/j.jff.2022.104975
Heidari H, Abdollahi M, Khani S, Nojavan F, Khani S. Effect of Alpinia officinarum extract on reproductive damages in streptozotocin induced diabetic male rats. J Diabetes Metab Disord. 2021; 20(1): 77–85. https://doi.org/10.1007/s40200-020-00711-0
Fajri M, Ahmadi A, Sadrkhanlou R. Protective effects of Equisetum arvense methanolic extract on sperm characteristics and in vitro fertilization potential in experimental diabetic mice: An experimental study. Int J Reprod Biomed. 2020; 18(2): 93–104. https://doi.org/10.18502%2Fijrm.v18i2.6415
Sukhikh S, Babich O, Prosekov A, Kalashnikova O, Noskova S, Bakhtiyarova A, et al. Antidiabetic Properties of Plant Secondary Metabolites. Metabolites. 2023; 13 (4): 513-539. https://doi.org/10.3390/metabo13040513
Devi S, Kaur N, Kumar P. In vitro and in vivo evaluation of antidiabetic potential and drug-herb interactions of Euphorbia neriifolia in streptozotocin-induced diabetes in rats and it’s in vitro antioxidant studies. Food Chem Adv. 2023; 2: 1-9. https://doi.org/10.1016/j.focha.2023.100199
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