تحديد طفيلي Leishmania tropica باستخدام تقنية Nested-PCR وبعض عوامل الضراوة في محافظة ذي قار، العراق
الشريط الجانبي للمقالة
محتوى المقالة الرئيسي
الملخص
تعد اللشمانيا الجلدية هي واحدة من الامراض المتوطنة في العراق، وهي تسبب من مشاكل صحية واسعة النطاق فضلا عن كونها أحد الامراض الغير مسيطر عليها. الدراسة الحالية هدفت الى تشخيص أنواع اللشمانيا المسببة للآفات الجلدية بين المرضى في محافظة ذي قار- جنوب العراق، كذلك لتحديد بعض عوامل الضراوة لطفيلي L. tropica. تضمنت هذه الدراسة ثلاث مستشفيات وهي الحسين التعليمي، سوق الشيوخ العام والشطرة العام في المحافظة للفترة من بداية شهر كانون الأول 2018 ولنهاية شهر أيلول 2019. تم جمع العينات من 80 مريض يعانون من الإصابة بداء اللشمانيا الجلدية ومن كلا الجنسين وبأعمار متنوعة، مواقع سكن مختلفة في المحافظة وآفات جلدية مفردة ومتعددة. استخدمت تقنية Nested-PCR لتضخيم جين kDNA لتشخيص أنواع اللشمانيا فضلا عن استخدام تقنية Conventional-PCR في التحري عن تواجد بعض جينات الضراوة. بينت نتائج الدراسة تواجد نوعين من الطفيلي في منطقة الدراسة. وبينت نتائج الترحيل الكهربائي لجين kDNA وجود 65 عينة موجبة لداء اللشمانيا الجلدية وبحجم 750bp للـ L. tropica و560bp للـ L. major. سجل طفيلي L. tropica نسبة بلغت 57.5% وكان النوع الأكثر شيوعا بينما سجل طفيلي L. major نسبة بلغت 23.75% حيث ظهرت بمستوى اقل. لم يلاحظ وجود فروق معنوية عند المقارنة بين إصابات الذكور والاناث، في حين بينت نتائج التحليل الاحصائي وجود فروق معنوية عند المقارنة بين المجاميع العمرية. كشفت الدراسة الحالية وجود جينات الضراوة LPG1, GP63, CPA and PPG1 في كل العزلات لطفيلي L. tropica وبنسبة تواجد بلغت 100%. كذلك كشفت الدراسة عن ان طفيليL. tropica هو النوع الرئيسي المسبب لداء اللشمانيا الجلدي في محافظة ذي قار، وجينات الضراوة هي ضرورية ومهمة لإمراضية الطفيلي.
Received 8/12/2019
Accepted 24/8/2020
تفاصيل المقالة
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كيفية الاقتباس
المراجع
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