Purification and Characterization β - lactamase produce from local isolate Klebsiella pneumonia

محتوى المقالة الرئيسي

Essam F. Al-Jumaily
Zuher A. S. Al-Taeei
Munatha A. Al-Safar

الملخص

Beta-lactamase was purified from local isolate Klebsiella pneumonia by several steps included precipitation with ammonium sulphate at 20-40% saturation, DEAE- ion exchange chromatography and gel filtration on Sephacryl S-200 column. The obtained purification fold and recovery were 32.66; 47.04% respectively. The characterization of the purified beta-lactamase showed that the molecular weight was about 4000 daltons as determined by gel filtration.Purified enzyme had an optimal pH of 7 for activity and an optimal stability between pH 6.5-7.5, results shows that the optimal temperature appear to be 35 ? C .During storage the enzyme retained 72% at -20 ? C and retained 25% of the activity at the same period at 4 ? C.

تفاصيل المقالة

كيفية الاقتباس
1.
Purification and Characterization β - lactamase produce from local isolate Klebsiella pneumonia. Baghdad Sci.J [انترنت]. 1 مارس، 2009 [وثق 24 ديسمبر، 2024];6(1):50-6. موجود في: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/956
القسم
article

كيفية الاقتباس

1.
Purification and Characterization β - lactamase produce from local isolate Klebsiella pneumonia. Baghdad Sci.J [انترنت]. 1 مارس، 2009 [وثق 24 ديسمبر، 2024];6(1):50-6. موجود في: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/956

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