Study the effect of Camellia sinensis alcoholic extract against Gram negative bacteria isolated from eye infections ( conjunctivitis)

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Sundus Adil Naji

Abstract

The present study examined the effect of alcoholic extract of Camellia sinensis against Gram negative bacteria isolated from eyes infections (conjunctivitis), 25 specimens (39.68%) gave positive bacterial culture from a total of 63 samples. The bacteria detected includes Pseudomonas aeruginosa  (12.69%), Enterobacter spp. (7.93%), Escherichia coli (6.34%), Moraxella spp. (4.76%), Proteus mirabilis (4.76%) and Klebsiella spp. (3.17%).


        The activity of the alcoholic extract of Camellia sinensis was also evaluated in the present study. The inhibitory ability of Camellia sinensis alcoholic extract against bacterial isolates at concentration (10,20,40,80,100)% showed the biggest average of inhibition zones diameters (25,23,23,17,15,13) mm at 100% concentration of P. aeruginosa, E.coli, Moraxella spp., Enterobacter spp., Klebsiella spp. and Proteus mirabilis isolates respectively.


        Different antibiotics were also used to compare their activities with the activity of Camellia sinensis alcoholic extracts.

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1.
Study the effect of Camellia sinensis alcoholic extract against Gram negative bacteria isolated from eye infections ( conjunctivitis). Baghdad Sci.J [Internet]. 2010 Jun. 6 [cited 2024 Oct. 19];7(2):1047-52. Available from: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/11926
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article

How to Cite

1.
Study the effect of Camellia sinensis alcoholic extract against Gram negative bacteria isolated from eye infections ( conjunctivitis). Baghdad Sci.J [Internet]. 2010 Jun. 6 [cited 2024 Oct. 19];7(2):1047-52. Available from: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/11926

References

Tarabishy, A.B. And Jeng, B.H. 2008. Bacterial conjunctivitis a review for internists Cleveland. Clinic journal of medicine. 75 (7):507-512.

Micallef, C. and Cuschiei, P. 2001. Ocular infections due to contaminated solution. Ophthalmologica. 21 (5): 337-342.

Trivastava, U. and Kolhapure, S.A. 2004. Evaluation of efficacy and safety ophthacare eye drops in acute and chronic conjunctivitis. The antiseptic.101 (6):211-217.

Brooks, G.F., Butel, J.S. and Morse, S.A.2001. Medical Microbiology. 22th. McGraw-Hill, Singapore.pages 818.

Finegold, S.M. and Baron, F.A. 1990.Baily and Scotts diagnostic Microbiology. Saunders, 8th ed, Philadelphia.pages 990.

Fobes, B.A., Saham, D.F. and Weissfeld, A.S. 1998. Diagnostic Microbiology,10th ed. Baily and Scotts. Mosby company. U.S.A.pages 1031.

Deshmukh, S.D. and Borle, M.N. (1975). Studies on the insecticidal properties of indigenous plant products. India,J.ENT.37(1):11-18.

Bauer,A.W. ; Kriby , W.M.M.; Sherris ,J.C. and Truck , M. 1966 . Antibiotic susceptibility testing by a standardized single method. AM.J . Clinic . Pathiol. 43(19): 493. 496 .

Locatelli, C.I., Kwitko, S. and Simonetti, A.B.2003. Conjunctival endogenous microbiola in patients submitted to cataract surgery. Brazilian journal of Microbiology. 1517 (34): 203-209.

Ikigai, H.,Nakae, T.,Hara, Y. and Shimamura, T. 1903. Bacteriocidal catechins damage the lipid bilayer. Biochim biophys. Acta. 1147(98): 132-136.

Jain, M.R.1990. Diagnosis and management of ocular inflammations. Znded Juypee broth. India.pages 223.

Joklik, W.K., Wilett, H.P., Amos, D.B. and Wilfert, C.M. 1992. Zinsser Microbiology. 20th ed. Appleton and lange. U.S.A.pages 875.

Yu, J., Wu, S. and Jia, H. 2001. Study on antimicrobial susceptibility of bacterial causing neonatal infections (1987-1998). Singapore. Med. J. 42(3):167-110.

AL-Saimary, I.E. 2007. A study of antibacterial activity of plant extracts on bacterial pathogens isolated from eyes infections (conjunctivitis). The internet journal of Microbiology. 4(1): 1.8.

Chen, C.J. and Starr, C.E. 2008. Epidemiology of Gram negative conjunctivitis in neonata intensive care unit patients. AmJ. Ophthalmology. 45 (6): 966-970.

Toda, M., Okabo, S., Hiyoshi, R. and Shimamura, T. 2008. The bactericididal activity of tea and coffee. Letters in applied Microbiology. 8(4):123-125.

Tiwari, R.P., Bharti, S.K., Kaur, H.D., Dikshit, R.P. and Hoodal, G.S. 2005. Synergistic antimicrobial activity of tea and antibiotics. Indian. J. Med. Res. 122(39): 80-84.

Mabe, K., Yamda, M., Oguni, I. and Takahashi, T.1996. In vitro and invivo activities of the catechins against Helicobacter pylori. Antimicrob. Agent. Chemother.43(18):1788-1791.

Michalczyk, M. and Zawislak, A. 2008. The effect of tea infusions on the proliferation of selected bacterial important for the human intestinal tract. Acta sientiarum polonorum. 7 (1): 59-65.

Khalaf, N.A., Shakya, A.K.,Othman, A.A., AL-Agbar, and Farah, H.2008. Antioxidants activity of some common plants. Turk. J.Biol.32(6):51-55.

Friedman, M.2007. Overview of antibacterial, antitoxin, antiviral and antifungal activities of tea flavonoids and teas flavonoids and teas. Molecular nutrition and food research. 51 (1): 116-134.

Iwalokun, B.A., Usen, U.A and Olukoya, D.K. 2007. Comparative phytochemical evalution, antimicrobial and antioxidant properties of Pleurotus ostreatus. African journal of Biotechnology. 6 (15): 116-134

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